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采用常规蒸汽爆破以及酸催化蒸汽爆破对甘蔗渣进行预处理,利用扫描电镜(SEM)对比观察这两种汽爆方法对残渣表面结构形态的影响,对比分析汽爆液的糖类、汽爆残渣组分,最后通过纤维素酶解试验评价汽爆残渣的酶解性能。结果表明,常规蒸汽爆破和酸催化蒸汽爆破均能提高蔗渣原料的酶解性能,但是常规蒸汽爆破需要在较高的处理压力(>2.0MPa)下才能明显水解半纤维素,破坏纤维素的天然结晶结构,显著提高蔗渣的酶解性能。酸催化蒸汽爆破只需很低的汽爆压力(0.7MPa)就能充分水解蔗渣半纤维素,而且对蔗渣纤维天然结构的破坏程度也要大得多,残渣的酶解率达到49.1%,比常规同一蒸汽爆破压力条件下的酶解率(25.1%)几乎高出一倍。酸催化汽爆残渣的酶解性能随汽爆压力的提高而改善,从半纤维素到木糖的收率则随压力的升高而降低,采取两步蒸汽爆破法,将可以同时获得高的木糖收率和高的纤维素酶解效率。
Conventional steam explosion and acid-catalyzed steam explosion were used to pretreat bagasse. SEM was used to observe the influence of these two steam explosion methods on the structure of the residue surface. Contrast analysis of the sugars, steam explosion residue Components, and finally enzymatic hydrolysis evaluation of steam explosion residue by cellulase hydrolysis test. The results showed that both conventional steam explosion and acid-catalyzed steam explosion could improve the enzymatic hydrolysis performance of bagasse raw materials. However, conventional steam explosion requires significant hydrolysis of hemicellulose at higher treatment pressures (> 2.0 MPa), destruction of natural cellulose Crystal structure, significantly improve the enzymatic hydrolysis performance of bagasse. Acid catalyzed steam explosion can fully hydrolyze bagasse hemicellulose with a very low steam explosion pressure (0.7 MPa), and the degree of destruction to the natural structure of bagasse fiber is also much greater. The residue hydrolysis rate reaches 49.1% Conventional steam explosion pressure under the conditions of the hydrolysis rate (25.1%) almost doubled. The enzymolysis performance of acid-catalyzed steam explosion residue improved with the steam explosion pressure, and the yield from hemicellulose to xylose decreased with the increase of pressure. By two-step steam explosion method, the high Xylose yield and high cellulolytic efficiency.