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目的 :评价基因重组干扰素γ( IFN-γ)对呼吸道合胞病毒感染 Th1/Th2 亚群功能的调节作用。方法 :将 40例 RSV毛细支气管炎患儿随机分为 IFN-γ治疗观察组 2 0例及治疗对照组 2 0例。 1 5例健康婴儿为正常组。用 EL ISA方法检测患儿血清中细胞因子IFN- γ、IL- 4水平。经 IFN- γ治疗后 7～ 1 0 d复查 IFN- γ、IL- 4水平。结果 :与正常组相比RSV毛细支气管炎患儿血清中 IFN- γ、IL- 4水平均有降低 ,其中 IFN- γ水平下降非常明显( P<0 .0 1 ) ,IFN- γ/IL- 4比值降低 ( P<0 .0 5 )。经 IFN- γ治疗后 ,IFN- γ治疗观察组 IFN- γ水平明显升高 ,IFN- γ/IL- 4比值升高 ,IFN- γ治疗观察组及治疗对照组 IL- 4水平均没有明显变化。提示 :婴幼儿 RSV毛细支气管炎患儿存在 Th细胞免疫功能状态紊乱 ,主要表现为Th1功能下降 ;Th2 功能相对增强。经 IFN- γ治疗后 ,IFN- γ/IL- 4比值升高 ,Th1/Th2 失衡得到纠正 ,但未发现 IFN-γ对 IL - 4有明显的抑制作用。
Objective: To evaluate the regulatory effect of recombinant interferon gamma (IFN-γ) on Th1 / Th2 subsets in respiratory syncytial virus infection. Methods: Forty infants with RSV bronchiolitis were randomly divided into treatment group (20 cases) and control group (20 cases). Fifteen healthy infants were normal group. ELISA method was used to detect serum IFN-γ, IL-4 levels in children. IFN-γ, IL-4 levels were examined 7 ~ 10 days after IFN-γ treatment. Results: Serum levels of IFN-γ and IL-4 in children with RSV bronchiolitis were significantly lower than those in the normal control group (P <0.01), IFN- γ / IL- 4 ratio decreased (P <0.05). After treatment with IFN-γ, the level of IFN-γ in IFN-γ treatment group was significantly increased, and the ratio of IFN-γ / IL-4 was increased. There was no significant change in IL-4 level between IFN-γ treatment group and control group . Tip: infants with RSV bronchiolitis in children with Th cellular immune function disorders, mainly as a decline in Th1 function; Th2 function was relatively enhanced. After treatment with IFN-γ, the ratio of IFN-γ / IL-4 increased and the imbalance of Th1 / Th2 was corrected. However, no significant inhibition of IL-4 was found by IFN-γ.