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目的建立检测抗精抗体的免疫斑点法,并与ELISA法进行比较。方法收集20份正常精液,混合,洗涤,混悬于0.5%NP-40 Tris-HCl缓冲液中,制备精子膜抗原,辣根过氧化物酶标记SPA,制备HRP-SPA,建立免疫斑点法检测抗精抗体,并对两种方法检测结果进行比较。结果免疫斑点法检测正常生育组30对夫妇共60例,其中阳性者1例为男性,阳性率为3.3%;检测不孕夫妇116对,共232例,阳性患者51例,阳性率为22.0%,其中男方占23例,阳性率为19.8%,女方占28例,阳性率为24.1%;检测习惯性流产夫妇53对,共106例,阳性患者16例,阳性率为15.1%,其中男方占2例,阳性率为3.8%,女方占14例,阳性率为26.4%。两种方法检测结果一致或差异不显著。结论免疫斑点法是一种比较稳定和敏感的方法,重复性、特异性好,结果易于判断,可应用于临床检验。
Objective To establish an immunoblot assay for the detection of anti-sperm antibodies and compare them with ELISA. Methods 20 normal semen samples were collected, mixed, washed and suspended in 0.5% NP-40 Tris-HCl buffer to prepare sperm membrane antigen and horseradish peroxidase-labeled SPA to prepare HRP-SPA. Anti-sperm, and the results of two methods were compared. Results In the normal fertility group, 30 couples and 60 couples were detected by immunofluorescence assay. Among them, 1 was male and the positive rate was 3.3%. A total of 116 pairs of infertile couples were detected, 232 were positive and 51 were positive. The positive rate was 22.0% , Of which 23 were male, the positive rate was 19.8%, the woman accounted for 28 cases, the positive rate was 24.1%; 53 pairs of habitual abortion were detected in 106 pairs of positive patients in 16 cases, the positive rate was 15.1%, of which male accounted 2 cases, the positive rate was 3.8%, the woman accounted for 14 cases, the positive rate was 26.4%. The two methods test results were the same or not significant difference. Conclusion Immunoturbidimetry is a stable and sensitive method with good repeatability and specificity and easy to judge the results. It can be used in clinical tests.