目的 :探讨CKLF基因与CKLFSF1基因间序列 (CCS)的调控作用。方法 :运用PCR技术扩增CCS ,并将此片段插入含有荧光素酶 (luciferase)报告基因载体 pGL3 basic ,以及 pGL3 SV4 0启动子中 ,构建受其调控的荧光素酶报告基因载体。应用脂质体介导基因转染技术 ,将 4种重组质粒转染Hela细胞 ,进行瞬时表达分析。结果 :在pGL3 basic和 pGL3 basic CCS质粒中的报告基因luciferase均无表达 ,但将CCS片段插入至 promoter上游后 ,荧光素酶活性增加近 1倍。 结论 :CKLF与CKLFSF1基因间的序列不具有启动子活性 ,但该序列中却可能存在调控其下游基因表达的顺式增强子元件 Objective: To investigate the regulation of CKLF gene and CKLFSF1 intergenic sequence (CCS). Methods: CCS was amplified by PCR and inserted into the luciferase reporter gene vector pGL3 basic and pGL3 SV40 promoters to construct a luciferase reporter gene vector. Four kinds of recombinant plasmids were transfected into Hela cells by liposome-mediated gene transfection, and transient expression analysis was carried out. Results: No reporter gene luciferase was expressed in the pGL3 basic and pGL3 basic CCS plasmids. However, luciferase activity was almost doubled after the CCS fragment was inserted into the promoter. CONCLUSION: The sequence between CKLF and CKLFSF1 does not have promoter activity, but cis-enhancer elements regulating the downstream gene expression may exist in this sequence