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气味结合蛋白(odorant binding proteins,OBPs)在昆虫嗅觉识别中起着重要的作用,尤其是在运输外界脂溶性气味分子通过嗅觉感器淋巴液到达嗅觉受体(olfactory receptors,ORs)的过程中发挥关键作用。明确OBPs在昆虫同外界进行信息交流过程中的作用有利于阐明昆虫嗅觉识别的机制,同时可为利用干扰昆虫嗅觉识别来进行害虫防治奠定理论基础。本研究克隆了一个绿盲蝽Apolygus lucorum(Meyer-Dür)气味结合蛋白AlucOBP7基因(GenBank登录号:JQ675724),并进行了原核表达,以1-NPN为荧光探针采用荧光竞争结合实验研究了AlucOBP7蛋白和10种棉花挥发物及6种性信息素类似物的结合能力。结果表明:在10种棉花挥发物中,AlucOBP7能够和2-己酮及水杨酸甲酯有效结合,结合常数分别为55.13μmol/L和28.26μmol/L。在6种盲蝽性信息素类似物中,4-氧代-反-2-己烯醛和AlucOBP7具有较强的结合能力,结合常数为23.14μmol/L。丁酸乙酯、丁酸丁酯及己酸己酯也能够和AlucOBP7有效结合,但结合能力中等,结合常数分别为30.58,39.26和35.81μmol/L。初步推测,AlucOBP7可能是绿盲蝽性信息素结合蛋白(pheromone binding proteins,PBPs),并在感受性信息素和植物挥发物的过程中发挥双重功能。
Odorant binding proteins (OBPs) play an important role in insect olfactory recognition, especially in the transport of liposoluble odor molecules to the olfactory receptors (ORs) through olfactory receptors’ lymph. Key role. To clarify the role of OBPs in the exchange of information between insects and the outside world is helpful to elucidate the mechanism of olfactory recognition in insects and lay a theoretical foundation for pest control using insect olfactory recognition. AlucOBP7 (GenBank accession number: JQ675724) was cloned from a Apolygus lucorum (Meyer-Dür) odorant binding protein, and prokaryotic expression was performed. Fluorescent competitive binding assay using 1-NPN as a fluorescent probe was used to study the effects of AlucOBP7 Protein and 10 cotton volatiles and 6 sex pheromone analogs. The results showed that AlucOBP7 could bind effectively to 2-hexanone and methyl salicylate with the binding constants of 55.13μmol / L and 28.26μmol / L, respectively. Of the 6 species of opsin pheromones, 4-oxo-trans-2-hexenal and AlucOBP7 have strong binding capacity with a binding constant of 23.14 μmol / L. Ethyl butyrate, butyl butyrate and hexyl hexanoate were also able to bind effectively to AlucOBP7, but with moderate binding capacity, the binding constants were 30.58, 39.26 and 35.81 μmol / L, respectively. It was preliminarily speculated that AlucOBP7 may be pheromone binding proteins (PBPs) that play a dual role in sensory pheromones and plant volatiles.