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目的:观察复方汉防已对人成纤维细胞增殖和凋亡及合成层粘蛋白的影响,探讨其抗肝纤维化的机制。方法:采用细胞培养法,于细胞培养液中加入不同浓度的药物,再培养48h,行流式细胞仪检测细胞 Fas 基因表达,部分细胞涂片进行 AgNOR 染色,观察 AgNOR 计数变化,培养上清用放免法测定层粘蛋白含量。结果:正常人成纤维细胞 AgNOR 计数为2.12±0.50个核,Fas 基因表达率为15.42%±6.20%,层粘蛋白含量为1.46±0.47mg/L;加入复方汉防已组 AgNOR 计数明显下降,Fas 表达率增加,培养上清层粘蛋白含量减少。结论:复方汉防已能抑制成纤维细胞的增殖和增加凋亡,使合成层粘蛋白减少,这可能是其抗肝纤维化机理之一。
OBJECTIVE: To observe the effects of compound Hanfang on the proliferation and apoptosis of human fibroblasts and the synthesis of laminin, and to explore the mechanism of anti-hepatic fibrosis. METHODS: Cell culture method was used to add different concentrations of drugs to cell culture medium and cultured for 48 hours. Fas gene expression was detected by flow cytometry. AgNOR staining was performed on some cell smears to observe changes in AgNOR counts and culture supernatants. Radioimmunoassay was used to determine the laminin content. Results: The AgNOR count of normal human fibroblasts was 2.12±0.50 nuclei, the expression rate of Fas gene was 15.42%±6.20%, and the laminin content was 1.46±0.47 mg/L. The AgNOR counts in the compound Hanfang group had significantly decreased. The expression rate of Fas increased and the content of mucin in the culture supernatant was reduced. Conclusion: Compound Hanfang can inhibit the proliferation of fibroblasts and increase the apoptosis, and reduce the mucin synthesis layer, which may be one of the mechanisms of anti-hepatic fibrosis.