ATHK1基因调节拟南芥渗透胁迫信号转导过程

来源 :植物生理与分子生物学学报 | 被引量 : 27次 | 上传用户:fanmw960
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以拟南芥ATHK1基因T-DNA插入所产生的缺失突变体和野生型WS(Wassilewskija)生态型为材料,分析了它们在生理和基因表达方面的差异。结果表明突变体的离体叶片失水率明显大于野生型;在30%PEG-6000胁迫后,野生型和ATHK1突变体的细胞膜离子外渗率比胁迫前分别增加了50%和80%。PEG胁迫48 h时突变体的萎蔫程度明显大于野生型WS。以上结果说明ATHK1突变体的抗渗透胁迫能力低于野生型,即ATHK1基因参与了拟南芥适应逆境的调节反应。利用DDRT-PCR技术研究二者在PEG胁
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