论文部分内容阅读
目的:对药用黄芪的遗传多样性进行研究,为黄芪的保护利用提供参考。方法:采用随机扩增多态DNA(RAPD)方法对15份黄芪基因组进行扩增,所获数据通过NTSYS-pc v2.1和POPGENE v1.3进行分析。结果:从47条引物中筛选出12条条带清晰且重复性好的引物用于实验和统计分析,共扩增出85个位点,多态性位点占78.16%。聚类分析显示,所有供试黄芪可明显聚为三类。结论:本研究中来自四个省份的黄芪具有较高的遗传多样性;材料间遗传距离的远近与其地理来源有一定相关性,其中四川理塘的野生黄芪与其他材料遗传距离较大,推测与其独特的生境有关。说明保护黄芪多样性应尽可能广泛收集不同来源地的种质资源。
Objective: To study the genetic diversity of medicinal Astragalus and provide a reference for the protection and utilization of Astragalus membranaceus. Methods: Fifteen RNAs of Astragalus membranaceus were amplified by random amplified polymorphic DNA (RAPD). The data were analyzed by NTSYS-pc v2.1 and POPGENE v1.3. RESULTS: Twelve of the 47 primers were screened for clear and reproducible primers for experimental and statistical analysis. A total of 85 loci were amplified, accounting for 78.16% of the total. Cluster analysis showed that all the tested Astragalus can be clearly grouped into three categories. Conclusion: Astragalus from four provinces in this study has a high genetic diversity; genetic distance between the material and its geographical origin has a certain relevance, including wild Astragalus and other materials in Litang, Sichuan genetic distance larger, speculated that its Unique habitat. Description Diversity of Astragalus should be collected as widely as possible Germplasm resources from different sources.