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应用酶组织化学方法,对实验性兔单纯疱疹性角膜炎角膜上皮中4种脱氢酶活性进行了测定。结果发现,接种单纯疱疹病毒Ⅰ型后第3天及第6天,角膜上皮中4种酶活性未见改变;第10天,琥珀酸脱氢酶、乳酸脱氢酶、葡萄糖-6-磷酸脱氢酶活性下降(P<0.05);第28天苹果酸脱氢酶活性下降(P<0.05),葡萄糖-6-磷酸脱氢酶活性仍未恢复。提示在单纯疱疹性角膜炎的不同病期,角膜上皮存在着这4种脱氢酶活性的不同改变。对这些酶活性改变的可能原因进行了讨论。
Enzymatic histochemistry was used to determine the activity of 4 dehydrogenases in the corneal epithelium of experimental herpes simplex keratitis. The results showed that there was no change in the activity of the four enzymes in the corneal epithelium on day 3 and 6 after inoculation of herpes simplex virus type Ⅰ. On the 10th day, succinate dehydrogenase, lactate dehydrogenase, glucose-6-phosphate (P <0.05). On the 28th day, the activity of malate dehydrogenase decreased (P <0.05) and the activity of glucose-6-phosphate dehydrogenase remained unchanged. Tip in the different stages of herpes simplex keratitis, there are corneal epithelial changes in the four kinds of dehydrogenase activity. Possible reasons for these changes in enzyme activity are discussed.