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目的:通过在神经缺损处局部回输体外分离培养纯化的淋巴细胞,了解此种方法促进面神经损伤修复的效果。方法:将20只Wistar大鼠的面神经颊支剪断并立即缝合(其余3支反折缝合)制成面神经损伤模型大鼠,将其分成淋巴细胞组和对照组,每组10只,每组再分成2周组和8周组。淋巴细胞组局部回输体外分离培养的外周血淋巴细胞,对照组作对照。于2周和8周测定面神经颊支-触须肌复合动作电位传导速度,辣根过氧化物酶(HRP)神经逆行示踪测定面神经核团的神经元阳性数目。结果:淋巴细胞组面神经颊支-触须肌复合动作电位传导速度8周时为0.64±0.07,与对照组(0.56±0.07)相比,差异有统计学意义(P<0.05)。HRP神经逆行示踪测定面神经核团神经元阳性数目,淋巴细胞组2周及8周与对照组同时间段相比差异均无统计学意义(均P>0.05)。结论:体外分离培养纯化的淋巴细胞在局部应用于神经损伤处对面神经再生修复可起一定的促进作用。
OBJECTIVE: To study the effect of this method on the repair of facial nerve injury through the isolation and culture of purified lymphocytes by local transfusion in vitro. Methods: The facial nerve buccal branch of 20 Wistar rats were cut and immediately sutured (the remaining three were sutured) to make the model of facial nerve injury. The rats were divided into lymphocyte group and control group, with 10 rats in each group Divided into 2 weeks group and 8 weeks group. Lymphocyte group partial return of cultured in vitro isolation of peripheral blood lymphocytes, the control group as a control. The facial nerve buccal-tendynaptic combined action potential conduction velocity was measured at 2 weeks and 8 weeks, and the number of neurons in facial nerve nuclei was determined by HRP retrograde tracing. Results: The conduction velocity of buccal-tendon complex action potential in the lymphocyte group was 0.64 ± 0.07 at 8 weeks, which was significantly different from that in the control group (0.56 ± 0.07) (P <0.05). There was no significant difference in the number of neurons in facial nerve nuclei between retrograde tracing with HRP neurons and the lymphocyte group at 2 weeks and 8 weeks (P> 0.05). CONCLUSION: Purified lymphocytes isolated and cultured in vitro may play a certain role in promoting facial nerve regeneration after local application to nerve injury.