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对大鼠肝、肺细胞液中脂氧合酶协同氧化 8种化学物质的活性进行比较研究。肝酶的适宜反应条件为Tris缓冲液、pH7 2、3 5mmol/L亚油酸和 2 13mg/L胞液蛋白 ;肺酶的适宜反应条件为Tris缓冲液、pH6 5、13 5mmol/L亚油酸和2 2 3mg/L胞液蛋白。抑制剂去甲二氢愈创木酸 (NDGA)对肝、肺脂氧合酶氧化联苯胺的反应均有明显抑制作用并呈剂量效应关系。除联苯胺外 ,四甲基联苯胺、邻苯三酚、愈创木酚、四甲基苯二胺、连氮双 ( 3-乙基苯并三唑 - 6 -磺酸 )、邻联 (二 )茴香胺和对苯二胺等 7种化学物质也被肝、肺脂氧合酶协同氧化。但肝酶活性仅相当于肺酶活性的 12 %～ 19%。认为出现这种差异的原因在于 :( 1)肝、肺组织中存在着不同的脂氧合酶同工酶 :( 2 )肝脂氧合酶所占胞液蛋白量的比例低、数量少。结果提示 :肝、肺脂氧合酶很可能是机体代谢某些具有苯环结构化学物质的重要途径
The activities of eight chemical substances synergistically oxidized by lipoxygenase in rat liver and lung cells were compared. The suitable reaction conditions of liver enzyme were Tris buffer solution, pH7 2,3 5 mmol / L linoleic acid and 2 13 mg / L cytosolic protein. The optimal reaction conditions of the enzyme for lung enzymes were Tris buffer, pH6 5, Acid and 2 2 3mg / L cytosolic protein. Inhibitors of nordihydroguaiaretic acid (NDGA) on the liver and lung lipoxygenase benzidine oxidation were significantly inhibited and dose-response relationship. In addition to benzidine, tetramethylbenzidine, pyrogallol, guaiacol, tetramethylphenylenediamine, azinobis (3-ethylbenzotriazole-6-sulfonic acid), vicinal B) Anisidine and p-phenylenediamine and other 7 kinds of chemical substances are also liver, lung oxygenase co-oxidation. However, liver enzyme activity is only equivalent to 12% to 19% of lung enzyme activity. The reason for this difference is that: (1) There are different lipoxygenase isozymes in liver and lung tissues: (2) The ratio of hepatic lipoxygenase to cytokines is low and the number is small. The results suggest that liver and lung lipoxygenases are probably important pathways for the metabolism of certain benzene ring-structured chemicals in the body