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目的:建立一测多评(QAMS)的方法用来测定酸枣仁中4种皂苷类成分的含量。方法:以酸枣仁皂苷A为参照物,采用HPLC法,以Kromasil C_(18)色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈-0.1%磷酸水溶液(30∶70);流速:1.0 ml·min~(-1);检测波长:280 nm;柱温:32℃;进样量:20μl。应用QAMS法的方法测定4种活性皂苷成分之间的相对校正因子,同时采用外标法测定该药材中4种成分的含量,得出的结果与QAMS法测定的结果进行比较。计算QAMS法测得含量计算结果(Wf)和外标法的测得含量测定结果(Ws)的相对误差,验证QAMS法的准确性。并对其重现性进行考察。结果:建立QAMS法用于测定酸枣仁中4种皂苷类成分的含量,并对16批酸枣仁药材进行测定,其计算值与测定值的差异较小(RSD<5%)。结论:该方法操作简单、专属性和重复性良好,在对照品缺乏的情况下,可以作为一个新模式用于酸枣仁药材的多成分定量。QAMS法测对酸枣仁的质量评价模式得到了验证,可用于酸枣仁药材的质量控制,并可为后续的QAMS研究提供参考价值。
Objective: To establish a multi-test (QAMS) method for the determination of four kinds of saponins in the content of saponins. Methods: The jujuboside A was used as a reference substance and was separated on a Kromasil C 18 column (250 mm × 4.6 mm, 5 μm) by HPLC. The mobile phase was acetonitrile-0.1% phosphoric acid solution (30:70) 1.0 ml · min ~ (-1); detection wavelength: 280 nm; column temperature: 32 ℃; injection volume: 20μl. The QAMS method was used to determine the relative correction factors of four active saponin components, and the contents of four components in the medicinal materials were determined by external standard method. The results were compared with the results of QAMS method. Calculate the relative error between the calculated results of the QAMS method (Wf) and the measured contents of the external standard method (Ws), and verify the accuracy of the QAMS method. And inspect its reproducibility. Results: The QAMS method was established to determine the content of four saponins in the wild jujube kernels. The 16 batches of wild jujube kernels were determined, and the difference between calculated and measured values was small (RSD <5%). Conclusion: The method is simple, specific and reproducible, and can be used as a new model for the quantitative determination of Chinese medicinal materials in the absence of reference substance. QAMS method for the quality evaluation of Spinosae has been verified, which can be used for quality control of Spinosae Radix and provide reference value for QAMS research.