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目的 建立肉瘤血行肺转移模型 ,探讨血管生成抑制剂TNP 470对肉瘤肺转移的形成及生长的影响。方法 筛选经反复小鼠腹腔注射 ,能持续引起小鼠血性腹水的S 1 80腹水癌细胞。按每鼠 1 .6× 1 0 6 / 0 .2ml经尾静脉接种于 1 5g左右的离乳幼鼠后随机分为对照组及TNP 470治疗组 ,每组 1 0只。接种后 2 4h起隔天皮下注射给药 ,第 1 5天处死小鼠 ,称肺湿重。肺组织切片 ,镜下计转移灶数。结果 经筛选的S 1 80肉瘤细胞尾静脉注射可成功建立小鼠血行肺转移模型。TNP 470 1 0 0mg/kg可有效减少小鼠肺转移模型中转移灶的数目并抑制转移灶的增大 ,但尚不能完全抑制转移灶的形成。结论 使用TNP 470等血管生成抑制剂可有效抑制肿瘤血行转移灶的形成和生长
Objective To establish a lung metastasis model of sarcoma and investigate the effect of angiogenesis inhibitor TNP 470 on the formation and growth of sarcoma lung metastasis. Methods S 180 ascitic carcinoma cells that persistently caused bloody ascites in mice were screened by repeated intraperitoneal injections. Rats were randomly divided into control group and TNP 470 treatment group (10 rats in each group) by intravesical inoculation of 1.6 × 10 6/0. Subcutaneously administered 24 hours after inoculation, mice were sacrificed on day 15, and the lungs were weighed. Lung tissue sections, the number of metastases under the microscope count. Results The mouse model of hematogenous lung metastasis was successfully established by tail vein injection of S180 sarcoma cells. TNP 470 1 0 0 mg / kg can effectively reduce the number of metastases in mice lung metastasis model and inhibit the increase of metastasis, but not completely inhibit the formation of metastases. Conclusions The use of TNP 470 and other angiogenesis inhibitors can effectively inhibit the formation and growth of tumor hematogenous metastases