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Objective To investigate the effect of estridiol and progesterone at different levels onnuclear factor-kappa-B (NF-κB) expression in mouse endometriumMethods Ovarioectmized mice were randomly divided into 9 groups, i.e. groups ofsham operation, of estridiol only, of progesterone only and of progesterone combinedwith estridiol. Mice in the experimental groups accepted subcutaneous injections ofestridiol or progesterone at different levels for one week. Their uteri were collected todetect the expression of NF-κB by immunohistochemical assay.Results Estridiol at a daily dose of 30 μg/kg and 100 μg/kg could significantly stimu-late the expression of NF-κB in mouse endometrium(P<0.01), but not in an obviousdose-dependent fashion (P>0.05). Progesterone alone could inhibit the expression ofNF-κB in cytoplasma (P<0.01). Combination of estridiol and progesterone could in-crease NF-κB expression both in cytoplasma and nuclear (P<0.01).Conclusion Eestridiol can up-regulate the expression of NF-κB in mouse endometrium,while progesterone exhibits down-regulation effect. This finding suggests that estridioland progesterone might modulate the endometrium receptivity by affecting NF-κBpathway.
Objective To investigate the effect of estridiol and progesterone at different levels on nuclear factor-kappa-B (NF-κB) expression in mouse endometrium Methods Ovarioectmized mice were randomly divided into 9 groups, ie groups ofsham operation, of estridiol only, of progesterone only and of progesterone combined with estridiol. Mice in the experimental groups accepted subcutaneous injections of estridiol or progesterone at different levels for one week. Their uteri were collected todetect the expression of NF- κB by immunohistochemical assay. Results Estridiol at a daily dose of 30 μg / kg and 100 (P> 0.05). Progesterone alone could inhibit the expression of NF-κB in cytoplasma (P <0.01), but not in an obvious dose-dependent fashion <0.01). Combination of estridiol and progesterone could in-crease NF-κB expression both in cytoplasma and nuclear (P <0.01) .Conclusion Eestridiol can up-regulate the expression o f NF-κB in mouse endometrium, while progesterone exhibits down-regulation effect. This finding suggests that estridioland progesterone might modulate the endometrium receptivity by affecting NF-κBpathway.