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植物名称:黑节草(Dendrobum candicum) 材料类别:种子在培养基上形成无菌苗后,将无菌苗的幼茎切成0.2—0.5cm的茎段作培养材料。培养条件:培养种子无菌苗是用RM的大量元素为基本培养基,每升附加BA0.2mg,NAA2mg,蔗糖3％,琼脂适量。培养室温度为20—25℃,光强 10001ux,每天光照8小时。生长与分化情况:黑节草的蒴果按常规作表面灭菌后,将蒴果内的种子播种在培养基上,待形成种子无菌苗后,再将无菌苗的幼茎茎段接种在分化
Plant Name: Dendrobum candicum Material Category: Seeds After the sterile seedlings are formed on the medium, the young stems of the sterile seedlings are cut into 0.2-0.5 cm stems for culture material. Culture conditions: sterile seed germination is the use of a large number of elements of RM as the basic medium, added BA0.2mg per liter, NAA2mg, sucrose 3%, the amount of agar. Incubation room temperature 20-25 ℃, light intensity 10001ux, light 8 hours a day. Growth and differentiation: After the capsules of P. vulgaris were surface-sterilized routinely, the seeds in the capsules were sown on the medium, and after the seedless aseptic seed was formed, the young stem segments of the aseptic seedlings were seeded on the differentiated