目的:比较微柱凝胶卡和传统试管法在新生儿溶血病(HDN)检测中的敏感性、准确性,以更好地指导临床应用。方法:用微柱凝胶卡和试管法分别对50例怀疑为ABO-HDN患者的血液进行直接抗人球蛋白试验、游离抗体试验和释放试验,并对结果进行比较。结果:3项检测中微柱凝胶卡分别检测出阳性23、32、43例,试管法分别检测出阳性21、29、37例,2者比较差异有统计学意义。结论:微柱凝胶的方法相比传统试管法,敏感度更高、操作更简便。 OBJECTIVE: To compare the sensitivity and accuracy of microcolumn gel cards and traditional test tube in the detection of neonatal hemolytic disease (HDN) to better guide the clinical application. Methods: Direct antiglobulin test, free antibody test and release test were performed on 50 patients suspected of being ABO-HDN using microcolumn gel cards and tubes respectively. The results were compared with each other. Results: Positive 23, 22 and 43 cases were detected by micro-gel cards in three tests respectively. There were 21,29 and 37 cases positive in test tube method, respectively. The difference between the two groups was statistically significant. Conclusion: The method of microcolumn gel is more sensitive and easier to operate than the traditional tube method.