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目的研究金雀异黄素(genistein,Gen)对中波紫外线(UVB)照射人角质形成细胞系HaCaT细胞所致氧化损伤的保护作用。方法体外培养HaCaT细胞,分成空白对照组(A组),25μmol/L金雀异黄素处理组(B组),UVB(30mJ/cm2)辐射组(C组),UVB辐射(30mJ/cm2)+25μmol/L金雀异黄素处理组(D组),MTT法检测细胞增殖,流式细胞仪检测活性氧(ROS)水平,生物化学法测定细胞超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性。结果 D组与C组相比,细胞存活率明显增加(C组62%,D组85%),细胞内ROS水平明显降低(C组237.21,D组120.57,抑制率为49%),细胞内SOD和GSH-Px水平明显升高[SOD:C组(180.55±8.34)nU/mL,D组(233.42±6.52)nU/mL;GSH-Px:C组(310.72±22.52)U,D组(380.98±29.50)U],两组之间的差别均有显著的统计学意义。结论 Gen能增加细胞在UVB照射后的存活率,增加细胞内的抗氧化酶活性,降低细胞内ROS水平,对UVB照射所致人角质形成细胞氧化损伤具有保护作用。
Objective To investigate the protective effect of genistein (Gen) on the oxidative damage induced by ultraviolet (UVB) radiation on human keratinocyte cell line HaCaT. Methods HaCaT cells were cultured in vitro and divided into blank control group (A group), 25μmol / L genistein group (B group), UVB radiation group (30mJ / cm2) + 25μmol / L genistein group (group D), MTT assay was used to detect the proliferation of cells, the level of reactive oxygen species (ROS) was detected by flow cytometry, the level of superoxide dismutase (SOD) Glycopeptide peroxidase (GSH-Px) activity. Results Compared with group C, group D showed a significant increase in cell viability (62% in group C, 85% in group D) and a significant decrease in intracellular ROS level (237.21 in group C, 120.57 in group D, and 49% in inhibition rate) The levels of SOD and GSH-Px were significantly higher in group C than those in group C [(180.55 ± 8.34) nU / mL for SOD: 233.42 ± 6.52 nU / mL for group D and 310.72 ± 22.52 for group C 380.98 ± 29.50) U], the differences between the two groups were statistically significant. Conclusion Gen can increase the survival rate of cells after UVB irradiation, increase intracellular antioxidant enzyme activity and reduce the intracellular ROS level, which can protect the oxidative damage of human keratinocytes induced by UVB irradiation.