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以电化学聚合法在石墨电极上获得聚间苯二胺膜（ＰＭＰＤ），并建立了快速、灵敏的膜质量检验方法：辣根过氧化物酶（ＨＲＰ）标记ＰＭＰＤ膜－邻苯二胺（ＯＰＤ）反应法，对聚合、活化等条件作了研究；进一步制成了检测乙肝表面抗原（ＨＢｓＡｇ）和检测鼠免疫蛋白（ＭＩｇＧ）的免疫电极，对血清样品作了检测．结果表明：２．５Ｖ电池电压下，于含０．１２ｍｏｌ／ＬＭＰＤ的１．２ｍｏｌ／ＬＨ２ＳＯ４中聚合２０ｍｉｎ，再以３０ｇ／Ｌ浓度的戊二醛活化４ｈ，所得ＨＲＰ标记电极及免疫电极重现性好；检测ＨＢ－ｓＡｇ和ＭＩｇＧ的线性范围分别为０．１～３．２ｍｇ／Ｌ及０．１～１０ｍｇ／Ｌ
Poly (m-phenylenediamine) membrane (PMPD) was obtained on graphite electrode by electrochemical polymerization. A fast and sensitive method for membrane quality testing was established. HRP-labeled PMPD membrane-o-phenylenediamine OPD) reaction, the conditions of polymerization and activation were studied, and further immunodelectrode for detection of hepatitis B surface antigen (HBsAg) and murine immunoglobulin (MIgG) were prepared. Serum samples were also tested. The results showed that the HRP labeled electrode and the immunorelectrode reproducibility were obtained after polymerization with 1.2 mol / L H2SO4 containing 0.12 mol / L MPD for 20 min at 2.5 V battery voltage and glutaraldehyde concentration 30 g / L for 4 h Good; linear range of detection of HB-sAg and MIgG were 0.1 ~ 3.2mg / L and 0.1 ~ 10mg / L