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目的:建立同时测定山玫胶囊中没食子酸、绿原酸、牡荆素葡萄糖苷、牡荆素鼠李糖苷、芦丁、牡荆素、金丝桃苷和槲皮素含量的高效液相色谱方法。方法:采用Agilent ZORBAX SB-C18色谱柱(4.6 mm×250 mm,5μm),以0.5%甲酸水溶液(A)-乙腈(B)-甲醇(C)-四氢呋喃(D)为流动相梯度洗脱,流速1.0 mL.min-1,检测波长260 nm、参比波长360 nm(0～15 min),检测波长370 nm、参比波长430 nm(15～65 min),柱温30℃。结果:没食子酸、绿原酸、牡荆素葡萄糖苷、牡荆素鼠李糖苷、芦丁、牡荆素、金丝桃苷和槲皮素的线性范围分别为9.38～300,11.2～360,18.7～600,31.8～1020,1.31～42.0,7.50～240,9.00～288,0.563～18.0μg.mL-1;r分别为0.9999,0.9995,0.9999,0.9999,0.9998,0.9998,0.9998,0.9999。平均加样回收率(n=6)分别为99.5%(RSD=1.6%),99.0%(RSD=1.2%),99.8%(RSD=1.7%),99.8%(RSD=1.3%),100.5%(RSD=1.8%),99.6%(RSD=1.5%),99.9%(RSD=2.0%),100.6%(RSD=1.1%)。结论:该分析方法准确可靠,重复性好,为更好地控制山玫胶囊内在质量提供科学依据。
OBJECTIVE: To establish a simultaneous determination of gallic acid, chlorogenic acid, vitexin glucoside, vitexin rhamnoside, rutin, vitexin, hyperoside and quercetin in ShanMei Capsules by HPLC method. METHODS: The mobile phase was eluted with an Agilent ZORBAX SB-C18 column (4.6 mm × 250 mm, 5 μm) using 0.5% formic acid in water (A) -acetonitrile (B) -methanol (C) The detection wavelength was 260 nm, the reference wavelength was 360 nm (0-15 min), the detection wavelength was 370 nm, the reference wavelength was 430 nm (15-65 min), and the column temperature was 30 ℃. Results: The linear ranges of gallic acid, chlorogenic acid, vitexin glucoside, vitexin rhamnoside, rutin, vitexin, hyperoside and quercetin were 9.38-300,11.2-360, 18.7 ~ 600,31.8 ~ 1020,1.31 ~ 42.0,7.50 ~ 240,9.00 ~ 288,0.563 ~ 18.0μg.mL-1; r were 0.9999,0.9995,0.9999,0.9999,0.9998,0.9998,0.9998,0.9999. The average recovery was 99.5% (RSD = 1.6%), 99.0% (RSD = 1.2%), 99.8% (RSD = 1.7%), 99.8% (RSD = 1.8%), 99.6% (RSD = 1.5%), 99.9% (RSD = 2.0%), 100.6% (RSD = 1.1%). Conclusion: The method is accurate and reliable with good repeatability and provides a scientific basis for better control of the intrinsic quality of Shan Mei capsule.