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The synthesis Of DNA, RNA and protein in L121O cells was inhibited by HHO7A in a dose-dependentand time-dependent way. HHO7A shOwed much stronger inhibitory effect on [3H]-thymidine incorporationthan that on [3H]-uridine or [3H]-tyrosine incorporation. The methods of shift of absorption spectrum,shift of fluorescence emission spectrum and excitation spectrum, and isotope technique were used to investigate the mechanism of actiOn of HHO7A on DNA synthesis. The results suggested thaT the action ofHHO7A on DNA synthesis was nOt probably due to direct damage to the DNA template, it might mainlyact by interfering with the metabolism of DNA.
The synthesis of DNA, RNA and protein in L121O cells was inhibited by HHO7A in a dose-dependent and time-dependent way. HHO7A shOwed much stronger inhibitory effect on [3H] -thymidine incorporation that that on [3H] -uridine or [3H] tyrosine incorporation. The methods of shift of absorption spectrum, shift of fluorescence emission spectrum and excitation spectrum, and isotope technique were used to investigate the mechanism of acti on of HHO7A on DNA synthesis. The results suggested thaT the action ofHHO7A on DNA synthesis was nOt probably due to direct damage to the DNA template, it might mainlyact by interfering with the metabolism of DNA.