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目的通过对蛋白免疫印迹法(WB)试验结果的观察,确定个体是否感染HIV以及HIV感染的状况。方法对60份抗-HIV初筛阳性血清使用蛋白免疫印迹法(WB)进行确认实验。结果在被检测样品中,HIV-1型确认试验阳性54例,占90%;HIV-1型确认试验阳性,且提示HIV-2阳性感染1例,占1.6%;HIV-1型确认试验弱阳性(不确定)3例,占5%;HIV-1型确认试验阳性2例,占3.3%。结论蛋白免疫印迹法(WB)试验确定个体有无HIV感染以及HIV感染情况提供依据。
Objective To determine whether an individual is infected with HIV and the status of HIV infection by observing the results of a Western blot (WB) test. Methods Sixty anti-HIV sera positive sera were confirmed by Western blotting (WB). Results Among the tested samples, HIV-1 confirmed positive test in 54 cases, accounting for 90%; HIV-1 confirmed positive test, and prompt HIV-2 positive infection in 1 case, accounting for 1.6%; HIV-1 confirmatory test is weak Positive (uncertain) in 3 cases, accounting for 5%; HIV-1 confirmed positive test in 2 cases, accounting for 3.3%. Conclusions Western blotting (WB) test provides a basis for determining whether an individual has HIV infection or not.