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镉(Cd)是烟草(Nicotiana tabacum L.)生长的非必需元素,但容易在烟草体内残留,危害其生长发育。本实验设定0~50 mg·L-17个梯度浓度的Cd2+,以MS+1.0mg·L-16-BA+0.5 mg·L-1NAA为基本培养基,诱导产生烟草叶片愈伤组织。待45 d愈伤组织丰富之后,每间隔3 d测定一次烟草愈伤组织内抗氧化系统相关酶活性、活性氧水平及丙二醛含量。结果表明:短期(45~51 d)Cd2+胁迫可导致烟草愈伤组织内活性氧水平升高,抗氧化酶系统活性增强,但长期(51~57 d)高浓度Cd2+胁迫导致抗氧化系统活性下降,膜质过氧化程度加重。
Cadmium (Cd) is an indispensable element for the growth of tobacco (Nicotiana tabacum L.), but easily remains in the tobacco and endangers its growth and development. In this experiment, Cd2 + concentration gradient of 0 ~ 50 mg · L-17 was set and MS + 1.0 mg · L-16-BA + 0.5 mg · L-1 NAA was used as the basic medium to induce tobacco leaf callus. After 45 days of callus enrichment, anti-oxidant system-related enzyme activity, reactive oxygen species and malondialdehyde content in tobacco callus were determined every 3 days. The results showed that short-term (45-51 d) Cd2 + stress led to the increase of reactive oxygen species (ROS) and the activity of antioxidant enzymes in tobacco callus, but long-term (51-57 d) Cd2 + stress led to the decrease of antioxidant activity , The degree of membranous hyperpensity increased.