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目的:观察原白头翁素衍生物(溴甲基呋喃酮)作用于人乳腺癌MCF-7细胞后Livin的表达率的变化及其意义。方法:用体外培养法培养人乳腺癌MCF-7细胞株,采用免疫细胞化学法检测Ki-67抗原和Livin蛋白表达情况。结果:原白头翁素衍生物体外对乳腺癌细胞有抗增殖作用,免疫细胞化学法分析表明原白头翁素衍生物能下调Ki-67抗原和Livin的表达。结论:原白头翁素衍生物对人乳腺癌MCF-7细胞有抑制作用,其机制可能与Livin下调相关。
OBJECTIVE: To observe the change of expression rate of Livin and its significance in the human breast cancer MCF-7 cells after using the original anion derivative (bromomethyl furanone). Methods: Human breast cancer cell line MCF-7 was cultured in vitro and the expression of Ki-67 antigen and Livin protein was detected by immunocytochemistry. RESULTS: The original Pulsatillae derivatives had anti-proliferative effects on breast cancer cells in vitro. Immunocytochemical analysis showed that the original Pulsatillae derivatives could down-regulate the expression of Ki-67 antigen and Livin. CONCLUSION: The original Pulsatillae derivative inhibits human breast cancer MCF-7 cells, and its mechanism may be related to down-regulation of Livin.