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环孢菌素A(CsA)与光敏交联剂 4 苯甲酰苯甲酸 (BBa)溶于丙酮 ,在紫外光照下 ,通过光化学反应在CsA残基侧链上引入活泼的羧基。纯化后的CsA BBa共轭物在水溶性碳二亚胺偶联剂的作用下与聚 L 赖氨酸载体发生偶联 ,获得免疫抗原。该方法更完整的保留了CsA的特征结构。选用具有更大氨基密度和较低免疫原性的聚 L 赖氨酸作载体 ,所得偶联效率更高 ,平均分子量为 4 0 0 0 0的PL载体上偶联 13 1- 17 4个CsA分子。
Cyclosporine A (CsA) and photosensitive crosslinker 4 (BBa) were dissolved in acetone and activated carboxyl groups were introduced into the side chains of CsA residues by photochemical reaction under ultraviolet light. The purified CsA BBa conjugate is coupled to the poly (L-lysine) carrier under the action of a water-soluble carbodiimide coupling agent to obtain an immunogen antigen. This method preserves the more complete CsA signature. The use of poly L-lysine with a greater amino group density and lower immunogenicity as a carrier resulted in a more efficient coupling with 13 17 A- CsA molecules on a PL vector with an average molecular weight of 40,000 .