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目的 动态观察血管形成素样蛋白3(angiopoietin-like protein 3,ANGPTL3)在阿 霉素大鼠肾病模型肾组织中表达水平的时相变化及细胞定位情况,探讨其与微小病变型肾病综 合征蛋白尿及高脂血症之间的关系。方法 给予大鼠一次性尾静脉注射阿霉素建立微小病变型 肾病综合征大鼠模型。采用免疫组织化学染色和斑点-ELISA,检测阿霉素注射后第7天、第14 天、第21天和第28天大鼠肾组织ANGFTL3的表达及分布。分析其与24 h尿蛋白量及血清胆 同醇(Cho)和甘油三酯(TG)的关系。结果(1)阿霉素注射后第14天开始出现大量蛋白尿(P<0.01), 血清白蛋白明显减少(P<0.01),Cho(P<0.01)和TG(P<0.05)明显增高,以上各项指标均在 第28天达高峰。(2)免疫组化定量分析显示阿霉素注射后第14天时,肾小球中ANGPTL3免疫 组化指数增高至同一时间点对照组的1.42倍(P<0.05);第21天时,为同一时间点对照组的 2.48倍(P<0.01),在肾小管中的表达也明显增高,免疫组化指数为同一时间点对照组的2.04 倍(P<0.01);到第28天,其在肾小球和肾小管中的表达均达最高水平,肾小球的免疫组化指 数为同一时间点对照组的3.28倍(P<0.01),肾小管的免疫组化指数为同一时间点对照组的 2.5倍(P<0.01)。(3)ANGPTL3在肾小球中的表达部位主要位于足细胞胞浆,此外,也可见于?
OBJECTIVE: To observe the temporal changes and cell location of angiopoietin-like protein 3 (ANGPTL3) in the adriamycin-induced nephropathy model and to investigate the relationship between the expression of angiopoietin-like protein 3 The relationship between urine and hyperlipidemia. Methods One-time intravenous injection of doxorubicin in rats was used to establish a rat model of minimal change nephrotic syndrome. Immunohistochemical staining and dot-ELISA were used to detect the expression and distribution of ANGFTL3 in the kidney of rats on the 7th, 14th, 21st and 28th day after doxorubicin injection. The relationship between 24 h urinary protein and serum Cho (Cho) and triglyceride (TG) was analyzed. Results (1) Massive proteinuria (P <0.01), serum albumin (P <0.01) and Cho (P <0.01) and TG The above indicators reached their peak on the 28th day. (2) Quantitative immunohistochemical analysis showed that on the 14th day after doxorubicin injection, the immunohistochemical index of ANGPTL3 in glomerulus was 1.42 times higher than that of the control group at the same time point (P <0.05); on the 21st day, the same time (P <0.01). The expression in renal tubules was also significantly increased, the immunohistochemical index was 2.04 times that of the control group at the same time point (P <0.01). On the 28th day, The expression of glomerular and tubule reached the highest level, the glomerular immunohistochemical index was 3.28 times that of the control group at the same time point (P <0.01), and the tubular immunohistochemical index of the same time point control group 2.5 Times (P <0.01). (3) The expression of ANGPTL3 in glomeruli mainly located in the podocyte cytoplasm, in addition, can also be seen in?