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目的 探讨脂肪酸合酶抑制剂对大肠癌细胞NF κB活性的抑制作用。方法 用脂肪酸合酶抑制剂(FASI) 浅蓝菌素处理大肠癌LoVo细胞株 ,用凝胶电泳迁移率法 (EMSA)检测NF κB活性。结果 实验分别用不同浓度浅蓝菌素 (10 -9～ 10 -5M )处理LoVo细胞 12h后 ,可抑制NF κB活化 ,其抑制程度呈剂量依赖性递减 ,与对照组相比较浅蓝菌素 (10 -9～ 10 -5M )处理LoVo细胞 12h后NF κB条带扫描像素积分比分别为 0 .787,0 .45 1,0 .379,0 .338,0 .32 2。结论 浅蓝菌素处理人大肠癌LoVo细胞后 ,NF κB活化程度随浅蓝菌素的剂量递增而递减。提示浅蓝菌素对LoVo细胞NF κB活性的具有抑制作用。
Objective To investigate the inhibitory effect of fatty acid synthase inhibitors on NF κB activity in colorectal cancer cells. Methods Colon cancer LoVo cell lines were treated with fatty acid synthase inhibitor (FASI) cerulenin, and NF κB activity was detected by electrophoretic mobility shift assay (EMSA). Results After treated with different concentrations of cerulenin (10 -9～10 -5M) for 12 h, LoVo cells inhibited the activation of NF κB, and the inhibition degree was dose-dependently decreased. Compared with the control group, cerulenin ( The integral ratios of NF κB band scanning pixels in LoVo cells after 12h treatment were 0. 787, 0. 45 1, 0 .379, 0 .338, 0.332 2 after 10 -9～ 10 -5M. Conclusion After cerulenin treatment of human colorectal cancer LoVo cells, the degree of NFκB activation decreases with increasing dose of cerulenin. It is suggested that cerulenin inhibits the activity of NFκB in LoVo cells.