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目的:探讨同型半胱氨酸(Hcy)对结直肠癌和肝癌增殖的影响。方法:收集结直肠癌、肝癌和健康人血浆204份测定Hcy。再检测Hcy对HepG2和HT29增殖的影响。结果:癌症组Hcy水平与健康组比较有统计学差异(P<0.05)。HepG2在4和2 mmol/L Hcy处理组与未处理组比较OD值增高(P<0.05);0.5和0.25 mmol/L处理组与未处理组比较OD值减低(P<0.05)。HT29在4 mmol/L Hcy处理组与未处理组比较OD值增高(P<0.05)。HepG2经0.5和0.25 mmol/L Hcy处理,DNA凝胶电泳均未形成梯状图形。HepG2和HT29经4和1 mmol/L Hcy处理,S期细胞构成比与未处理组比较均无统计学差异(P>0.05)。HepG2经0.25 mmol/L Hcy处理未检出亚二倍体峰。结论:Hcy达一定浓度时有促进肿瘤细胞增殖的潜能。
Objective: To investigate the effect of homocysteine (Hcy) on the proliferation of colorectal cancer and liver cancer. Methods: Colorectal cancer, liver cancer and healthy plasma 204 were collected to determine Hcy. The effect of Hcy on the proliferation of HepG2 and HT29 was also tested. Results: The Hcy level in cancer group was significantly higher than that in healthy group (P <0.05). The OD value of HepG2 in 4 and 2 mmol / L Hcy treatment group was significantly higher than that in untreated group (P <0.05). The OD value of HepG2 in 0.5 and 0.25 mmol / L treatment group was significantly lower than that in untreated group (P <0.05). HT29 in 4 mmol / L Hcy treatment group compared with the untreated group OD value increased (P <0.05). HepG2 was treated with 0.5 and 0.25 mmol / L Hcy, no DNA gel electrophoresis ladder pattern. HepG2 and HT29 treated with 4 and 1 mmol / L Hcy had no significant difference in S phase cell composition ratio compared with untreated group (P> 0.05). HepG2 treated with 0.25 mmol / L Hcy did not detect sub-diploid peak. Conclusion: When Hcy reaches a certain concentration, it has the potential to promote tumor cell proliferation.