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1992年在玉米族远缘杂交组合 34 0 2 F3(丹 34 0× 4 0 3- 2 )中首次发现带标记性状的基因雄性不育 ( GMS)材料。遗传分析结果表明 ,不育性受一对隐性基因控制。当不育株 ( A)与可育株 ( B)进行兄妹交 ,育性分离比例接近 1∶ 1;而可育株 ( B)自交的后代 ,可育株与不育株的分离比例为 3∶ 1。连锁遗传分析结果证明 ,不育基因 ( ms°)与标记性状基因 ( plf)紧密连锁 ,未发现有交换现象 ,它们可能位于同一染色体同一位点上。经过不育株五代兄妹交已选出几个高产核不育两用系。通过测交筛选出一个强优势高产恢复系 HR- 1。利用高产核不育系与强优恢复系配制出两个超高产玉米杂交种 ;小区( 0 .0 0 66hm2 )产量分别为 72 .0 kg和 78.1kg,比对照掖单 13号增产 18.6%和 2 8.7%。本文还讨论了带遗传标记的高产核不育两用系的应用前景 ,并提出进一步研究 ms°- plf基因的设想。
In 1992, the male sterile male sterile (GMS) material with marker trait was first found in the maize hybrid group 34 0 2 F3 (Dan 34 0 × 4 0 3- 2). Genetic analysis showed that sterility was controlled by a pair of recessive genes. When male sterile lines (A) and male fertile lines (B) were crossed with siblings, the fertility segregation ratio was close to 1: 1. For male fertile lines (B), the segregation ratio of fertile and sterile lines was 3:1. The results of linkage analysis showed that MS (ms °) was closely linked with the marker gene (plf), and no exchange phenomenon was found. These genes may be located at the same site of the same chromosome. After five generations of sterile strains of siblings have selected several high-yielding sterile lines. Screening out a strong and powerful high yield restorer line HR-1. Two high-yielding maize hybrids were constructed using high yielding nuclear and strong restorer lines. The yields of plots (0. 0 66hm2) were 72.0 kg and 78.1 kg, respectively, which were 18.6% higher than that of the control Yedan 13 2 8.7%. This article also discussed the application prospects of high yielding genic male sterile lines with genetic markers and proposed the idea of further studying ms ° - plf gene.