论文部分内容阅读
目的：以普通紫外分光光度计代替自动生化分析仪等贵重仪器，建立血清谷胱甘肽Ｓ－转移酶（ＧＳＴ）测定方法．方法：选择最佳酶反应条件和操作程序，排除非酶反应干扰，提高测定的灵敏度和准确性．结果：观察了５０例正常人血清ＧＳＴ活性，ｘ±ｓ为４．６±３．１，正常小鼠与对乙酰氨基酚中毒鼠血清ＧＳＴ活性分别为３４．４±４．２和２１５７±５２４，差别非常显著（Ｐ＜０．０１），这些数据与文献中用自动或半自动生化分析仪所得结果基本一致．结论：本法检测血清ＧＳＴ活性简便、经济、可靠、易于推广应用．
OBJECTIVE: To establish a method for the determination of glutathione S-transferase (GST) by using ordinary UV spectrophotometer instead of expensive instruments such as automatic biochemical analyzer. Methods: Select the best enzyme reaction conditions and operating procedures to exclude non-enzymatic interference and improve the sensitivity and accuracy of the assay. Results: Serum GST activity was observed in 50 normal individuals with x ± s of 4.6 ± 3.1. Serum GST activity was 34.4 ± 4.2 and 2157 ± 524 in normal and acetaminophen-challenged mice, respectively , The difference is very significant (P <0.01). These data are basically the same as the results obtained from the automatic or semi-automatic biochemical analyzer in the literature. Conclusion: The method for detecting serum GST activity is simple, economical, reliable and easy to popularize.