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目的研究炎性相关基因IL-1F5(interleukin-1 factor 5)的3’非编码区microRNA(miRNA)靶序列单核苷酸多态性(SNP)与胃癌易感性关系。方法采用1∶1频数匹配病例-对照研究对研究对象进行基因分型,应用多因素条件logistic回归及多因素降维法(MDR)分析SNP与胃癌易感性的关系及其与环境因素的交互作用。结果 rs2472188位点GC基因型(OR=1.50,95%CI=1.13~1.99)和rs2515401位点CT基因型(OR=1.42,95%CI=1.09~1.86)是胃癌的易感基因;单体型分析显示,单体型1(CCGCA)(OR=2.08,95%CI=1.27~3.40)、单体型4(CTATA)(OR=1.98,95%CI=1.48~2.66)、单体型5(CTATC)(OR=10.13,95%CI=4.43~23.16)可增加胃癌发病风险,而单体型2(CTACA)(OR=0.18,95%CI=0.12~0.28)、单体型3(CTACC)(OR=0.37,95%CI=0.23~0.59)、单体型9(GCGTC)(OR=0.39,95%CI=0.27~0.58)可降低胃癌发病风险;具有rs2472188、rs2515401以及rs2515402组合的人群是胃癌发病风险的高危人群(OR=6.17,95%CI=4.61~8.36)。结论 rs2472188位点GC基因型、rs2515401位点CT基因型是胃癌的易感基因型,多位点联合作用对于胃癌的发生可能有协同作用。
Objective To study the relationship between SNP of 3 ’untranslated region (SNP) of microRNA (miRNA) gene and susceptibility to gastric cancer in interleukin-1 factor 5 (IL-1F5) Methods A 1: 1 frequency matched case-control study was used to genotype the subjects. Multivariate conditional logistic regression and multivariate dimensionality reduction (MDR) were used to analyze the relationship between SNP and gastric cancer susceptibility and its interaction with environmental factors . Results The genotypes of rs2472188 GC (OR = 1.50, 95% CI = 1.13-1.99) and rs2515401 CT genotype (OR = 1.42, 95% CI = 1.09-1.86) were the predisposing genes of gastric cancer. Analysis showed that patients with haplotype 1 (CCGCA) (OR = 2.08, 95% CI = 1.27-3.40), haplotype 4 (CTATA) (OR = 0.18, 95% CI = 0.12-0.28), and haplotype 3 (CTACC) (OR = 10.13,95% CI = 4.43-23.16) increased the risk of gastric cancer, (OR = 0.37, 95% CI = 0.23-0.59), and haplotype 9 (GCGTC) (OR = 0.39,95% CI = 0.27-0.58) could reduce the risk of gastric cancer. The population with rs2472188, rs2515401 and rs2515402 The risk of gastric cancer in high-risk groups (OR = 6.17, 95% CI = 4.61 ~ 8.36). Conclusion GC genotype at rs2472188 locus and genotype rs2515401 at CT loci are susceptible genotypes of gastric cancer. The combination of multiple sites may have synergistic effect on the occurrence of gastric cancer.