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目的:研究银翘柴桂颗粒对流感病毒FM1感染小鼠免疫功能的影响。方法:用甲1型流感病毒FM1鼠肺适应株感染小鼠,建立小鼠流感病毒性肺炎的生物模型,用不同浓度银翘柴桂颗粒溶液治疗后,用ELISA方法检测小鼠血清中IFN-γ及细胞因子IL-2、IL-6含量;中性红比色法测定腹腔巨噬细胞的吞噬能力。结果:银翘柴桂颗粒各剂量组均能明显上调小鼠腹腔巨噬细胞的吞噬能力(P<0.01),而中高剂量组能够提高IFN-γ、IL-2活性,同时抑制IL-6的活性(P<0.05)。结论:银翘柴桂颗粒可通过调节机体的免疫功能发挥抗病毒作用。
Objective: To study the effect of Yinqiao Chagu Granule on immune function in mice infected with influenza virus FM1. Methods: The mouse model of influenza virus pneumonia was established by infecting mice with influenza A virus type FM1 mouse lung-adapted strain. After being treated with different concentrations of Agkistrodon pubescentis particle solution, the levels of IFN- γ and cytokines IL-2, IL-6 content; Neutral red colorimetric determination of phagocytic capacity of peritoneal macrophages. Results: Yinqiao Chaigui granule could significantly up-regulate the phagocytic capacity of peritoneal macrophages in mice (P <0.01), while the medium-high dose group could increase the activity of IFN-γ and IL-2 and inhibit the expression of IL-6 Activity (P <0.05). Conclusion: Yinqiao chaigui granules can play an antiviral effect by regulating the body’s immune function.