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本文研究了不同光质对甜茶(Rubus suavissimus S.Lee)愈伤组织生长及甜茶甙(Rubusoside)产生的影响。愈伤组织在黑暗中培养28天可获得最大的生物量。与黑暗相比,白光、蓝光、红光、远红光及短红光均对细胞生长有一定抑制作用,其中蓝光抑制作用最强,其次为红光。但是蓝光促进甜茶甙的产生,在7天时,可获得高于在其它光质培养下的甜茶甙含量。黑暗下甜茶甙含量最低。为了获得较高的甜茶甙产量,甜茶愈伤组织分两步培养,在黑暗中培养28天,然后转移到蓝光下培养7天。
In this paper, the effects of different light qualities on callus growth and Rubusoside production in sweet tea (Rubus suavissimus S. Lee) were studied. Callus in the dark for 28 days to get the maximum biomass. Compared with the darkness, white light, blue light, red light, far red light and short red light all have a certain inhibitory effect on cell growth, of which blue light has the strongest inhibitory effect, followed by red light. Blu-ray, however, promoted the production of tachyosin, which at 7 days achieved higher levels of tachyosin than other light cultures. Dark glyphosate lowest content. In order to obtain higher yield of teurin, sweet calluses were cultured in two steps, cultured for 28 days in the dark and then transferred to blue light for 7 days.