仿生髓核组织工程材料支架对体外培养髓核细胞合成代谢的影响

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目的:观察自行设计构建的仿生髓核组织工程材料--CⅡ/HyA-CS(CHCS)支架对体外培养髓核细胞合成代谢的影响.方法:将体外培养的传1代髓核细胞接种于培养瓶内,置入CHCS支架,体外培养10d后,分别测定3H-脯氨酸掺入量、培养液中糖胺聚糖(GAG)含量、髓核细胞可凝集蛋白多糖(Aggrecan)、核多糖(Decorin)、二聚糖(Biglycan)的mRNA表达及Aggrecan的蛋白表达等.结果:实验组与对照组的3H-脯氨酸掺人量、培养液中GAG含量、髓核细胞Aggrecan、Decorin、Biglycan mRNA表达、Aggrecan蛋白表达等均无统计学差异.结论:CHCS支架对体外培养髓核细胞的合成代谢无明显抑制作用.
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