以3-巯丙基三甲氧基硅烷为偶联剂,将末端带有活性基团的聚N-异丙基丙烯酰胺(PNIPAM)键合到硅胶上,作为高效液相色谱固定相填料(Sil-PNIPAM)。以正己烷-异丙醇混合液为流动相,苯的衍生物为溶质对Sil-PNIPAM的正相色谱行为进行考察。结果表明该键合相在正相色谱模式下对带有供电子和/或者吸电子基团的化合物具有良好的色谱分离性能。研究表明,正相模式下该键合相的保留机理存在着氢键及π-π等相互作用机制。 Using 3-mercaptopropyltrimethoxysilane as the coupling agent, poly (N-isopropylacrylamide) (PNIPAM) with active groups at the end was bonded to silica gel and used as a stationary phase of high performance liquid chromatography -PNIPAM). The normal phase chromatographic behavior of Sil-PNIPAM was investigated with n-hexane-isopropanol mixture as mobile phase and benzene derivative as solute. The results show that the bonded phase has good chromatographic separation performance for compounds with electron-donor and / or electron-withdrawing groups in normal phase chromatography. The results show that the mechanism of hydrogen bond and π-π interaction exists in the retention mechanism of the bonded phase in the normal phase mode.