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目的和方法：应用双色免疫荧光流式细胞技术观察了肿瘤坏死因子（ＴＮＦα）对人外周血中性粒细胞（ＣＤ１５＋细胞）粘附分子ＣＤ１１α、ＣＤ１１ｂ、ＣＤ４９ｄ和ＣＤ６２Ｌ的诱生作用和氨茶碱对其影响。结果：不同浓度的ＴＮＦα可诱导中性粒细胞ＣＤ１１ａ和ＣＤ６２Ｌ的表达，对ＣＤ１１ｂ和ＣＤ４９ｄ无明显作用。不同剂量的氨茶碱对粘附分子的表达有一定作用。氨茶碱在７５ｍｇ／Ｌ浓度时增强ＣＤ１１ａ和ＣＤ６２Ｌ的表达；１２５ｍｇ／Ｌ时抑制其表达；２５０ｍｇ／Ｌ时抑制ＣＤ６２Ｌ的表达，对ＣＤ１１ａ无明显作用。结论：ＴＮＦα可调节中性粒细胞表面某些粘附分子的表达，氨茶碱对粘附分子的作用在临床应用上具有一定的指导意义。
Objective and Methods: The induction of tumor necrosis factor (TNFα) on the adhesion molecules CD11α, CD11b, CD49d and CD62L of human peripheral blood neutrophils (CD15 + cells) and the effect of aminophylline Impact on it. RESULTS: Different concentrations of TNFα induced the expression of CD11a and CD62L in neutrophils, but had no significant effect on CD11b and CD49d. Different doses of aminophylline on the expression of adhesion molecules have a role. Aminophylline enhanced the expression of CD11a and CD62L at the concentration of 75mg / L, inhibited the expression at 125mg / L and inhibited the expression of CD62L at 250mg / L, which showed no significant effect on CD11a. CONCLUSION: TNFα can regulate the expression of some adhesion molecules on the surface of neutrophils, and the effect of aminophylline on adhesion molecules has some guiding significance in clinical application.