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用50μmol/L GM_3处理J6-2细胞6天,经组化与细胞学检查,证实细胞沿单核巨噬细胞途径分化。以[~(32)P]Pi或[CH_3-~(14)C]胆碱冲激(Pulse)后,将洗净的细胞进行Folch分配。取下相进行薄层层析,再做放射自显影。结果表明:GM_3的处理能促进[~(32)P]Pi或[CH_3-~(14)C]胆碱向磷脂酰胆碱的参入,而抑制向其他磷脂组分的参入。用[CH_3-~(14)C]胆碱冲激的Folch分配上相含水溶性胆碱代谢物,经TLC,结果表明CM_3的处理使[CH_3-~(14)C]胆碱向CDP-胆碱的参入减少。本研究证实,GM_3能调节J6-2细胞的磷脂代谢,其调节机制值得研究。
J6-2 cells were treated with 50μmol / L GM_3 for 6 days. Histochemistry and cytology showed that the cells differentiated along the monocyte-macrophage pathway. After pulsing with [~ (32) P] Pi or [CH_3- ~ (14) C] choline, the washed cells were subjected to Folch partitioning. Take the phase thin layer chromatography, do autoradiography. The results showed that the treatment of GM_3 promoted the incorporation of [~ (32) P] Pi or [CH_3- ~ (14) C] choline into phosphatidylcholine and inhibited the incorporation of other phospholipid components. The results of TLC showed that the treatment of CM_3 [CH_3- ~ (14) C] choline to CDP-gall Alkali participation reduced. This study confirmed that, GM_3 can regulate phospholipid metabolism in J6-2 cells, and its regulatory mechanism is worth studying.