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目的比较瑞舒伐他汀和阿托伐他汀对新生SD大鼠心脏成纤维细胞(CFs)的抑制作用。方法采用新生SD大鼠CFs进行体外培养,分别给予不同剂量瑞舒伐他汀和阿托伐他汀进行干预。应用MTT法测定CFs细胞数,5-溴脱氧尿嘧啶法测定DNA合成,羟脯氨酸法测定胶原合成,流式细胞仪测定细胞增殖周期。结果瑞舒伐他汀和阿托伐他汀均可明显抑制新生SD大鼠CFs增殖、DNA合成和胶原合成。在相同体外药物浓度下,2药对成纤维细胞的抑制作用无明显差异。2药均可增加CFs处于G0/G1期的百分率,降低CFs处于S期的百分率。结论瑞舒伐他汀和阿托伐他汀均为有效的抗心脏纤维化药物,2种药对心脏成纤维细胞的抑制作用无明显差异。
Objective To compare the inhibitory effects of rosuvastatin and atorvastatin on cardiac fibroblasts (CFs) in neonatal SD rats. Methods Newborn SD rats CFs were cultured in vitro and were given different doses of rosuvastatin and atorvastatin for intervention. The number of CFs was measured by MTT assay, DNA synthesis by 5-bromodeoxyuridine assay, collagen synthesis by hydroxyproline assay, and cell proliferation cycle by flow cytometry. Results Rosuvastatin and atorvastatin could significantly inhibit the proliferation, DNA synthesis and collagen synthesis of CFs in neonatal SD rats. In the same in vitro drug concentration, 2 drugs on fibroblasts no significant difference in the role of inhibition. 2 drugs can increase the percentage of CFs in G0 / G1 phase, reduce the percentage of CFs in S phase. Conclusion Both rosuvastatin and atorvastatin are effective anti-cardiac fibrosis drugs, and there is no significant difference between the two drugs on cardiac fibroblasts.