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目的建立一种采用UPLC-MS/MS检测全血中磺胺嘧啶的方法。方法以HLB固相萃取柱提取血样,应用UPLC色谱法分离,MS/MS检测分析。结果该方法具有浓缩富集的效果,使血液中磺胺嘧啶的回收率高于102%,线性范围为10～1 000ng/mL,检测限0.21ng/mL(S/N≥3)。结论该方法灵敏度高,分析速度快,操作简便,可用于全血中磺胺嘧啶的定性、定量分析检验。
Objective To establish a method for the determination of sulfadiazine in whole blood by UPLC-MS / MS. Methods Blood samples were extracted with HLB solid phase extraction column and separated by UPLC and MS / MS. Results The method has the effect of concentration enrichment, the recovery of sulfadiazine in the blood is higher than 102%, the linear range is 10 ~ 1 000 ng / mL and the limit of detection is 0.21 ng / mL (S / N≥3). Conclusion The method has high sensitivity, fast analysis, easy operation and can be used for qualitative and quantitative analysis of sulfadiazine in whole blood.