【摘 要】
：
Three plant binary expression vectors-pNAR501,pNAR502 and pNAR503-were constructed,carrying fragments of exon2-exon3,5’partial deletion exonl and 5’partial de
【机 构】
：
State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanj
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Three plant binary expression vectors-pNAR501,pNAR502 and pNAR503-were constructed,carrying fragments of exon2-exon3,5’partial deletion exonl and 5’partial deletion exonl-exon2-exon3 ofPib gene driven by 35S.These three vectors were transformed into the japonica rice variety Nipponbare through agrobacterium-mediated transformation.More than 30 transgenic rice plants were obtained and confirmed by polymerase chain reaction (PCR),South hybridization and the hygromycin resistance test in seed germination of their progeny.A rice blast resistance test for in vitro leaves ofT0 transgenic plants in the tillering stage showed higher resistance to the races of E1,F1 and G1 office blast than that of the control Nipponbare.However,results of rice blast resistance test for seedlings of T1 transgenic plants in the 3- to 4-leaf stage were different.All T1 transgenic seedlings had a lower level of resistance to E1,F1and G1 races than that of the control Nipponbare.
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