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应用免疫组化和免疫电镜观察61例大肠腺癌组织中胃泌素表达,同时应用放射免疫法检测血清胃泌素水平,结果36例(59％)呈阳性反应,高分化癌阳性率(13/16,81.3％)高于中分化癌(21/40,52.5％)和低分化癌(2/5,40.0％)。胃泌素表达强度与分化程度和Dukes分期有关。胃泌素阳性颗粒弥漫分布于癌细胞浆。免疫电镜胶体金颗粒(PAG)主要分布于癌细胞内不同电子密度分泌颗粒、癌细胞间隙和微绒毛膜表面。揭示癌细胞胃泌素表达是大肠癌分化良好的标志。癌细胞形成分泌颗粒释放胃泌素。血清胃泌素水平在癌组织胃泌素强阳性表达的病例中增高,可反映癌组织中胃泌素水平变化。
Immunohistochemistry and immunoelectron microscopy were used to observe the expression of gastrin in 61 cases of colorectal adenocarcinoma, and serum gastrin levels were detected by radioimmunoassay. The results were positive in 36 cases (59%), and the positive rate of well-differentiated carcinoma was (13) /16,81.3%) were higher than those with moderately differentiated cancer (21/40, 52.5%) and poorly differentiated cancer (2/5, 40.0%). Gastrin expression intensity is related to the degree of differentiation and Dukes stage. Gastrin-positive granules are diffusely distributed in the cytoplasm of cancer cells. Immunoelectron microscopy colloidal gold particles (PAG) are mainly distributed in the different electron density secreting granules in the cancer cells, the gap between the cancer cells and the microvilli surface. Revealing cancer cell gastrin expression is a hallmark of good differentiation of colorectal cancer. Cancer cells form secretory granules that release gastrin. Serum gastrin levels are elevated in cases of strong gastrin-positive expression in cancer tissue, reflecting changes in gastrin levels in cancer tissues.