基因饰变技术在蛋白质工程中占有重要的地位。它不仅为基因、蛋白质的结构与功能关系的研究,而且也为治疗、临床检验,工业用酶的改良提供了重要的工具。一、基因饰变技术寡核苷酸定位诱变该法示如图1。①制备含目的基因的质粒或噬菌体的单股DNA;②把合成的寡核苷酸引物导入变异位点。这个寡核苷酸称为诱变引物(其中含 Genetic modification technology in protein engineering occupies an important position. It not only for gene, protein structure and function of the relationship between research, but also for the treatment, clinical testing, industrial enzymes provide an important tool for improvement. First, the genetic modification technology Oligonucleotide localization mutagenesis The method shown in Figure 1. ① Preparation of plasmid or phage containing the gene of interest single-stranded DNA; ② synthesis of oligonucleotide primers into the mutation site. This oligonucleotide is called a mutagenic primer (which contains