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方法:将接种了结直肠癌C26细胞的雄性BALB/C小鼠随机分为对照组和运动组。于接种细胞的第0,7,14,21,28和第35天于鼠尾静脉收集血清用于测定PGE2水平。每周测量肿瘤的大小,记录小鼠死亡时间。取部分肿瘤组织检测PGE2含量及测定肿瘤组织COX-2的表达。结果:运动组与对照组肿瘤体积均随时间而逐渐增加,对照组肿瘤体积的增加明显快于运动组。同时,对照组小鼠的荷瘤生存率明显低于运动组。与对照组相比,运动组肿瘤组织COX-2的表达,肿瘤组织匀浆液及血清中的PGE2含量均明显降低。结论:运动对C26结直肠癌荷瘤小鼠肿瘤的生长有明显的抑制作用。同时,对荷瘤小鼠的生存率也有明显的改善作用。运动抑制肿瘤组织COX-2的表达,降低血清及肿瘤局部组织的PGE2水平可能是产生这种作用的原因之一。
METHODS: Male BALB/C mice inoculated with colorectal cancer C26 cells were randomly divided into control and exercise groups. Sera were collected from the tail vein of rats at 0, 7, 14, 21, 28, and 35 days after seeding for PGE2 levels. Tumor size was measured weekly and the time of mouse death was recorded. PGE2 levels were measured in some tumor tissues and the expression of COX-2 in tumor tissues was measured. RESULTS: The volume of tumors in the exercise group and the control group increased gradually with time, and the tumor volume in the control group increased significantly faster than that in the exercise group. At the same time, the tumor-bearing survival rate of mice in the control group was significantly lower than that in the exercise group. Compared with the control group, the expression of COX-2 in the tumor tissue of the exercise group, and the PGE2 content in the tumor homogenate and serum were all significantly reduced. Conclusion: Exercise has significant inhibitory effect on the growth of tumor in C26 colorectal cancer-bearing mice. At the same time, the survival rate of tumor-bearing mice was also significantly improved. Exercise inhibits the expression of COX-2 in tumor tissues, and reducing serum and tumor tissue PGE2 levels may be one of the reasons for this effect.