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目的:观察黄灵颗粒对荷瘤小鼠免疫器官的调节作用及对细胞凋亡相关基因的影响。方法:将接种肝癌腹水瘤(H_(22))的昆明种小鼠给药后按规定处死,取脾脏及胸腺称重并计算胸腺指数和脾脏指数;按试剂盒说明进行免疲组化检测。结果:黄灵颗粒对H_(22)荷瘤小鼠的免疫器官无明显作用,肝癌小鼠肿瘤细胞bcl-2、P53蛋白均有降低作用。结论:黄灵颗粒的抑癌作用是通过抑制癌基因的表达而实现的,并且对免疫器官的保护作用明显优于化疗药物,有利于荷瘤机体的治疗。
Objective: To observe the regulatory effect of Huangling granule on immune organs of tumor-bearing mice and its effect on apoptosis-related genes. Methods: Kunming mice inoculated with hepatocellular carcinoma (H_ (22)) were sacrificed as required. The spleen and thymus were weighed and the thymus index and spleen index were calculated. The kit was tested for the absence of fatigue. Results: Huangling granule had no significant effect on the immune organs of H22 tumor-bearing mice, but decreased the expression of bcl-2 and P53 in tumor cells of HCC mice. Conclusion: The anti-tumor effect of Huangling granule is achieved by inhibiting the expression of oncogenes, and its protective effect on immune organs is obviously superior to chemotherapy drugs, which is good for the treatment of tumor-bearing body.