论文部分内容阅读
目的使用实时荧光定量PCR(qRT-PCR)法检测胃癌淋巴结的微转移情况,并探讨微转移的临床意义。方法收集我院2010年1～6月期间40例行胃癌根治术切除的281枚和10例行胃十二指肠溃疡手术切除的39枚,共计320枚淋巴结标本,以CEA、CK-19和CK-20为引物进行qRT-PCR检测其微转移情况,并分析微转移的临床病理特点。结果 40例胃癌患者中有28例(70.00%)、31枚(15.35%,31/202)淋巴结检测出有微转移。10例胃溃疡的39枚淋巴结标本,HE染色检测和qRT-PCR检测均为阴性。淋巴结微转移的阳性率与肿瘤分化程度、浸润深度和临床分期有关(P<0.05)。结论 qRT-PCR是检测胃癌淋巴结微转移敏感且特异的方法,对胃癌临床分期、判断预后以及治疗方案选择具有重要意义。
Objective To detect the micrometastasis of lymph nodes in gastric cancer by real-time fluorescence quantitative PCR (qRT-PCR) and to explore the clinical significance of micrometastasis. Methods A total of 320 lymph node specimens from 40 patients undergoing radical resection of gastric cancer in our hospital from January 2010 to June 2010 were collected, and 39 of them were resected with CEA, CK-19 and CK-20 as a primer for qRT-PCR detection of micrometastasis, and analyze the clinical and pathological features of micrometastasis. Results Twenty-eight cases (70.00%) of 40 gastric cancer patients and 31 (15.35%, 31/202) lymph nodes were detected with micrometastasis. The 39 lymph node specimens from 10 gastric ulcer patients were negative for HE staining and qRT-PCR. The positive rate of lymph node micrometastasis correlated with tumor differentiation, depth of invasion and clinical stage (P <0.05). Conclusion qRT-PCR is a sensitive and specific method for detecting lymph node micrometastases in gastric cancer. It has important significance for clinical staging, prognosis and treatment options.