目的 :应用免疫组化和核酸原位杂交技术检测人胚胎甲状腺的ＭＥＬ受体及其亚型 ,为进一步研究ＭＥＬ对甲状腺功能的调节作用提供证据。方法 :1 研究对象 :中期水囊引产人胎儿 ,取出甲状腺 ,立即用4%多聚甲醛 + 0 .1 %ＤＥＰＣ固定 ,石蜡包埋组织 ,4℃冰箱贮存待检测。 2 ＭＥ Objective: To detect the MEL receptors and their subtypes of thyroid gland by immunohistochemistry and in situ hybridization in situ hybridization, which may provide evidence for the further study on the regulatory effect of MEL on thyroid function. Methods: 1 Subjects: Indwelling human fetuses in the mid-term water sac, removing the thyroid gland, immediately fixed with 4% paraformaldehyde + 0.1% DEPC, paraffin-embedded tissue, stored in 4 ℃ refrigerator to be tested. 2 ME