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目的 探讨p2 7蛋白在宫颈癌中表达的意义及p2 7基因缺失、突变在宫颈癌发生中的作用。方法 采用链霉菌抗生素蛋白 -过氧化酶 (S -P)免疫组化法及聚合酶链反应 -单链构象多态性 (PCR -SSCP)分析法分别检测宫颈癌中p2 7基因的蛋白表达及其两个外显子的缺失、突变。结果 p2 7蛋白表达 ,18例正常宫颈上皮组织均为高表达 ,48例宫颈癌组织中 ,19% ( 9/48)高表达 ,81% ( 39/48)低表达 ,宫颈癌中高表达率明显低于正常宫颈组织 (P <0 .0 5 ) ;宫颈癌的分化程度中 ,Ⅰ级高表达 5 0 % ( 5 /10 )明显高于Ⅱ级 13% ( 3/2 4)及Ⅲ级 7% ( 1/14 ) (P <0 .0 5 ) ;伴有淋巴结转移的高表达4% ( 1/2 3) ,不伴淋巴结转移的高表达 32 % ( 8/2 5 ) ,差异有显著性 (P <0 .0 5 ) ;p2 7基因缺失、突变分析 ,17例宫颈癌及 8例正常宫颈组织均未发现一例外显子Ⅰa、Ⅰb及外显子Ⅱ缺失、突变。结论 p2 7与宫颈癌的发生发展、分化程度及淋巴结转移有关 ;宫颈癌中未发现p2 7基因缺失与突变 ;对p2 7蛋白进行检测为诊断宫颈癌及临床综合治疗提供参考。
Objective To investigate the significance of p27 in cervical cancer and the role of p27 in the development of cervical cancer. Methods The protein expression of p27 gene in cervical cancer was detected by S-P immunohistochemistry and PCR-SSCP method. The deletion of two exons, mutation. Results The expression of p27 protein was high in 18 cases of normal cervical epithelium and in 48 cases of cervical carcinoma, high expression was found in 19% (9/48), low expression in 81% (39/48), high expression in cervical cancer (P <0.05). Among the differentiation degree of cervical cancer, the grade Ⅰ high expression 50% (5/10) was significantly higher than that of grade Ⅱ 13% (3/2 4) and grade Ⅲ 7 % (1/14) (P <0.05); the high expression of lymph node metastasis was 4% (1/2 3), and the high expression of lymph node metastasis was 32% (8/2 5), the difference was significant (P <0.05). No deletion, mutation of exonⅠa, Ⅰb and exon Ⅱ were found in p27 gene, mutation analysis, 17 cases of cervical cancer and 8 cases of normal cervical tissue. Conclusions The expression of p27 in cervical cancer is related to the occurrence, differentiation and lymph node metastasis of cervical cancer. The deletion and mutation of p27 in cervical cancer are not found. The detection of p27 protein provides a reference for diagnosis of cervical cancer and clinical treatment.