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目的 :比较采用冷冻精子、新鲜精子、睾丸精子进行卵胞浆内单精子注射 (ICSI)治疗的结局。方法 :女方常规超排卵和经阴道B超引导下取卵 ,成熟卵子进行ICSI注射 ,比较采用冷冻精子组 (组Ⅰ )、新鲜精子组 (组Ⅱ )和睾丸精子组 (组Ⅲ )的受精率、卵裂率、临床妊娠率。结果 :组Ⅰ (冷冻精子 ICSI) 5 8个周期 :受精率为 78 3 % ,卵裂率为 92 0 % ,临床妊娠率为 48 2 % ;组Ⅱ (经皮穿刺睾丸取精 ICSI) 88个周期 :受精率为 74 5 % ,卵裂率为93 9% ,临床妊娠率为 3 1 7% ;组Ⅲ (新鲜精液 ICSI) 14 2个周期 :受精率为 71 9% ,卵裂率为 91 6% ,临床妊娠率为 2 9 4%。组Ⅰ临床妊娠率明显高于组Ⅱ与组Ⅲ (P <0 0 5 ) ;3组的受精率及卵裂率差异无显著意义 (P >0 0 5 )。结论 :精子冷冻复苏处理不影响ICSI的受精率和妊娠率。睾丸精子与新鲜精子ICSI可取得类似的临床效果
OBJECTIVE: To compare the outcomes of intracytoplasmic sperm injection (ICSI) with frozen sperm, fresh sperm, and testicular sperm. Methods: Female ovulation and mature ovum were injected with ICSI under routine superovulation and transvaginal ultrasonography. The fertilization rates of frozen sperm group (group Ⅰ), fresh sperm group (group Ⅱ) and testicle sperm group (group Ⅲ) were compared. , Cleavage rate, clinical pregnancy rate. Results: The ICSI of Group Ⅰ (frozen spermatozoa ICSI) was 58 cycles: fertilization rate of 78 3%, cleavage rate of 92 0%, clinical pregnancy rate of 48 2%; group Ⅱ (percutaneous transcutaneous testicular sperm retrieval ICSI) 88 Cycles: The fertilization rate was 74.5%, the cleavage rate was 93.9%, and the clinical pregnancy rate was 31.7%. The group Ⅲ (fresh semen ICSI) 14 2 cycles: the fertilization rate was 71.9% and the cleavage rate was 91 6%, the clinical pregnancy rate was 294%. The clinical pregnancy rate in group I was significantly higher than those in groups II and III (P <0.05). There was no significant difference in fertilization rate and cleavage rate among the three groups (P> 0.05). Conclusion: Sperm cryopreservation does not affect ICSI fertilization rate and pregnancy rate. Testicular sperm and fresh sperm ICSI can achieve similar clinical results