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目的探讨牛磺酸对染矽尘大鼠肺组织炎性细胞趋化的影响。方法 Wistar大鼠144只,随机分为对照组、染矽尘组和牛磺酸组,后2组气管内注入质量浓度为50 g/L的矽尘混悬液,牛磺酸组在实验开始前3 d即给予含质量分数为2.5%的牛磺酸饲料持续喂养,对照组气管内注入等体积灭菌生理氯化钠溶液。偏振光显微镜观察肺纤维化发生情况,行在体全肺灌洗术,血细胞计数仪测定支气管肺泡灌洗液细胞数。结果牛磺酸组第7、14、21和28天炎性细胞总数均低于染矽尘组,差异有统计学意义(P<0.05)。牛磺酸组第14、21天巨噬细胞数低于染矽尘组,差异有统计学意义(P<0.05)。牛磺酸组第21天中性粒细胞数低于染矽尘组,差异有统计学意义(P<0.05)。牛磺酸组第14、21天淋巴细胞数均低于染矽尘组,差异有统计学意义(P<0.05)。结论牛磺酸对矽尘诱导大鼠肺组织巨噬细胞、中性粒细胞、淋巴细胞的趋化具有抑制作用。
Objective To investigate the effect of taurine on chemotaxis of lung inflammatory cells in rats exposed to silica dust. Methods A total of 144 Wistar rats were randomly divided into four groups: control group, silica dust group and taurine group. The latter two groups were given intratracheal instillation of silica suspension with mass concentration of 50 g / L. Before the experiment On the 3rd day, the taurine feed containing 2.5% (mass fraction) of feed was fed continuously. The control group was injected intratracheally with equal volumes of sterile physiological sodium chloride solution. The occurrence of pulmonary fibrosis was observed under a polarized light microscope, and the number of bronchoalveolar lavage fluid cells was measured in whole body lung lavage and hemocytometer. Results The total number of inflammatory cells in the taurine group at 7th, 14th, 21st and 28th day were lower than that in the group with silica dust, the difference was statistically significant (P <0.05). The number of macrophages in the taurine group on the 14th and 21st days was lower than that on the silica dust group, with statistical significance (P <0.05). The number of neutrophils on day 21 in taurine group was lower than that on silica dust group, with statistical significance (P <0.05). The number of lymphocytes in the taurine group at 14 and 21 days was lower than that in the silica group (P <0.05). Conclusion Taurine can inhibit the chemotaxis of macrophages, neutrophils and lymphocytes in rat lung induced by silica dust.