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目的通过对华支睾吸虫感染兔模型的研究,了解有效治疗前后血清抗体的消长规律,为探讨特异性抗体检测用于疗效考核的价值提供参考。方法自感染的鱼体分离华支睾吸虫囊蚴,经口感染7只家兔,14周后给予吡喹酮治疗,使用SPA-ELISA法检测感染及治疗前后血清IgG抗体,观察针对华支睾吸虫粗抗原的抗体消长规律。结果7只实验兔均感染成功,抗体水平在感染后2～4周内显示不同程度的上升,至第4周均达较高水平,8～12周继续上升至峰值(0.143～0.307)。经过有效驱虫治疗,特异性抗体水平总体上呈现下降趋势,大部分兔(71.4%)在治疗5周后下降显著。到死亡或饲养结束,71.4%的实验兔A值降至0.1以下,为感染前的1.28～1.80倍。此外,解剖观察显示,完全驱虫治疗7或10个月后的3只兔肝仍可见病理性结节。结论华支睾吸虫感染后IgG抗体水平的上升较早,部分兔感染后约2周就有明显上升,使用高敏感度的抗体检测方法有助于对部分感染者进行早期诊断、早期治疗。有效驱虫治疗后,大部分实验动物显示抗体水平先快速后缓慢下降的规律,血清抗体滴度的动态观察具有一定的疗效考核价值。此外,本研究显示单纯驱虫治疗并不能使华支睾吸虫感染动物的肝脏病理改变很快恢复,提醒我们在防治工作中须重视该寄生虫寄生所引起的长期肝脏损害,将预防感染放在第一位的同时,对感染者的治疗应联合使用肝脏康复药物。
OBJECTIVE: To study the growth and decline of serum antibody before and after effective treatment of Clonorchis sinensis in rabbits and to provide reference for the value of specific antibody test in curative effect evaluation. METHODS: Clonorchis sinensis metacercariae were isolated from infected fish and seven rabbits were orally infected. After 14 weeks, praziquantel was administered. Serum IgG antibodies were detected by SPA-ELISA before and after treatment. Antibodies to flukes rough antigen rule. Results Seven rabbits were infected successfully. The level of antibody increased to some extent in 2 ~ 4 weeks after infection, reached the highest level in the 4th week and continued to rise to the peak (0.143 ~ 0.307) in 8 ~ 12 weeks. After effective de-worming treatment, the level of specific antibodies showed a general downward trend. Most rabbits (71.4%) showed a significant decrease after 5 weeks of treatment. At the end of the death or feeding, 71.4% of rabbits A value dropped below 0.1, 1.28 to 1.80 times before infection. In addition, anatomical observations showed that pathological nodules were still visible in 3 rabbits at 7 or 10 months after complete deworming treatment. Conclusion The IgG antibody level of Clonorchis sinensis increased earlier and some rabbits increased significantly after about 2 weeks of infection. The detection of antibodies with high sensitivity may be helpful for the early diagnosis and early treatment of some infected persons. Effective deworming treatment, most of the experimental animals showed a rapid decline in the first antibody level after the law, the dynamic observation of serum antibody titers have a therapeutic value of the assessment. In addition, this study showed that simple deworming treatment can not make the liver pathological changes of animals infected with Clonorchis sinensis quickly restore reminded us in the prevention and control work must pay attention to parasitic parasites caused by long-term liver damage, will prevent infection on At the same time, the treatment of infected persons should be combined with liver rehabilitation drugs.