论文部分内容阅读
目的为了探讨淋病奈瑟菌耐药谱与质粒谱之间的相互关系。方法我们对本地区１９９１年、１９９６年各５０株标本进行了耐药谱、质粒谱分析。以琼脂扩散法测定其１２种抗生素敏感性；碱裂解加溶菌酶法进行质粒抽提。结果１９９１年分离的标本以同时耐２种抗生素者为主，耐药谱型２４种，质粒谱型５种。１９９６年分离的标本以同时耐３或４种抗生素者为主，耐药谱型３４种，质粒谱型１０种。结论不同时期分离的标本耐药谱、质粒谱变化较大。同一时期分离的标本尽管耐药谱型较分散，但质粒谱型相对稳定。说明本地区淋病奈瑟菌耐药菌株的流行主要是染色体突变所致。
Objective To explore the relationship between drug resistance spectrum and plasmid spectrum of Neisseria gonorrhoeae. Methods We carried out the drug resistance spectrum and plasmid spectrum analysis of 50 strains from 1991 to 1996 in this area. The agar diffusion method was used to determine the sensitivity of 12 antibiotics. Lysis of alkaline solution and lysozyme method were used for plasmid extraction. Results The specimens isolated in 1991 were mainly resistant to two antibiotics at the same time. There were 24 resistance spectrum and 5 plasmid patterns. The samples isolated in 1996 were mainly resistant to 3 or 4 antibiotics at the same time. There were 34 drug resistance spectrum and 10 plasmid spectrum. Conclusion The drug resistance spectrum and the plasmid spectrum of the specimens isolated in different periods have great changes. Although the spectrum of drug resistance was more dispersed during the same period, the plasmid profile was relatively stable. This shows that the prevalence of Neisseria gonorrhoeae strains in this area is mainly due to chromosomal mutation.